Detection of Rutin test in Tea - (Qualitatively)

Detection of Rutin

Qualitatively detected

Introduction

 Rutin is an example of flavanones/flavanoids which should give you the impression of being an antioxidant substance, it is found in a number of fruits and vegetables, it has a wide range of health benefits such as being anticarcinogenic and anti-inflammatory effects.

Chemical structure of Rutin
quercetin-3-rutinoside

Rutin is composed of a disaccharide ( Rhamnose + glucose) glycosylated with a quercetin group ( which is a plant polyphenol compound )

Protein disulfide isomerase is an essential enzyme in protein folding that is synthesized in the endoplasmic reticulum of our cells & platelets and its function is to catalyze the formation and breakage of disulfide bonds - the function of such enzyme is sort of a mixed blessing where performing its job inside the cell is a good thing but when it goes outside the cell it becomes lethal where it can clots proteins which can then accumulate inside the blood vessels, blocking them, ultimately causing Blood clots.

Beside other compounds tested, Rutin was found to be the perfect one to be the inhibitor of Protein disulfide isomerase (PDI) and at the same time it's structure prevent it from entering the cell or otherwise, it would roam around inhibiting any nearby enzyme, so it was said to be anti-thrombotic activity.

Foods rich in Rutin

• Onion
• Tea
• Apples
• Citrus fruits
• Buckwheat

Tools

1. Graduated cylinder (preferably 50ml.)
2. 2 Droppers 
3. Ferric chloride solution
4. 2 test tubes, one kept as a control and the other the test.
5. Source of flame

Procedures

1. Weight 1 gram of tea (rutin source)
2. Add 50ml. of water in a boiler, and leave it till it starts to boil
3. The onset of boiling put your 1 gram tea.
4. Leave it to boil for five minutes.
5. After five minutes of boiling, cool the mixture.
6. Transfer 1ml. of your tea solution in each of your 2 test tubes.
7. Add few drops of ferric chloride solution to the test tube and leave the control tube blank.
8. Record your observation.

Result

• An olive-green color should be formed as a positive result for the detection of Rutin in tea.

A positive result can be seen in the upper (test) tube comparing it to the control tube

References

• Food chemistry 15306
• https://news.harvard.edu/gazette/story/2012/05/flavonoid-compound-can-prevent-blood-clots/
• https://harvardmagazine.com/2012/09/curbing-clots
• https://www.healthline.com/health/potential-benefits-of-rutin#diet
• https://www.medicinenet.com/rutin/supplements-vitamins.htm
• http://www.phytochemicals.info/phytochemicals/rutin.php
• https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3739031/

Thanks a lot for reading, I hope you find it informative

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