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Showing posts with label penicillium digitatum. Show all posts
Showing posts with label penicillium digitatum. Show all posts

Sunday, 23 April 2017

Todd has been sick! Short tale

Growing Penicillium digitatum on Todd ( an orange )

Under general environment 

As you can see the green parts indicates the development of conidiophores
and as well as the fruiting bodies which carry the spores

Under general environment, an orange named Todd was left for a while infront of a window a couple of days and symptoms of fungal infection was seen ( white color ) as the beginning, it indicates the sprouting of spores to hyphae which then forms a network of mycelium and later on forms the fruiting bodies which carry the spores at that time it becomes green in color.

The mold scientific name is Penicillium digitatum

The main causal agent of fruit-rot to citrus fruits ( e,g, orange as in our case) the green mold disease, also it is a major post-harvest disease which should be taken into consideration when harvesting our crop.

A mesophilic fungus which prefers temperatures between 15*C-45*C, of Division: Ascomycota 

Following a strict means of clean and sanitized storage, handling and packaging can ensure the least infection, removal of any infected plants quickly before the mold grows and infects other fruits comes in handy, applying specific fungicides in particular concentrations and much more from chemical and biological controls can be found in the following website:http://www.plantwise.org/KnowledgeBank/Datasheet.aspx?dsid=39570

Culturing it, due to the limited resources I have since of my inability to afford tools for culture media, I have used Gelatin, I bought a strawberry Jelly, prepared it and inoculated a sample of the fungi in it and after 5 days leaving the petri dish in room temperature, results were:
My very first culture media

This was Todd after a week from the last picture shown
Needed to be contained
Observing the mold structure under optical microscope







The following clip ( I DON'T OWN IT - Copyrights goes to its owner) shows a fastened footage of the mold on a lemon: https://www.youtube.com/watch?v=W1Bzi7T8-HQ
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Saturday, 24 December 2016

Microscope reveals


Home ant (1) 
(2)



Antenna of the ant

Close look to this ant's leg

Plant cells of an onion - stained by iodine ( the only stain I had at the current moment)

A close look to the plant cells of an onion

Cells extracted from the inner lining of my cheeks also stained by iodine





Wing of Culex mosquito
Stinging apparatus of Culex moquito



Legs of Culex mosquito

Mouthparts of Musca domestica (house fly)

Wings of Musca domestica (house fly)

Rhizopus stolonifer (bread mould) sporangium + sporangiophore

Rhizopus stolonifer
Gram-positive Monobacillus 
Gram-positive Monobacillus

Penicillium digitatum
Peniciliium digitatum


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